Difference between revisions of "Polymerase chain reaction"

Revision as of 20:52, 22 March 2020

This article is a direct transclusion of the Wikipedia article and therefore may not meet the same editing standards as LIMSwiki.

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-The '''polymerase chain reaction''' ('''PCR''') is a scientific technique in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.
+{{wikipedia:Polymerase chain reaction}}
-===Polymerase chain reaction system===
+==Notes==
+This article is a direct transclusion of [https://en.wikipedia.org/wiki/Polymerase_chain_reaction the Wikipedia article] and therefore may not meet the same editing standards as LIMSwiki.
-Modern PRC systems not only amplify a piece of DNA, but also make accurate qualitative and quantitative detections of nucleic acids and genotyping in real-time.
-==References==
-<references />
 <!---Place all category tags here-->
 [[Category:Scientific techniques]]

v t e Polymerase chain reaction techniques
Procedure	Initialization Cycle Denaturation Annealing Elongation Final elongation Final hold
Polymerase	Klenow T4 and T7 Taq Tth Pfu Vent Pwo Tli Tfu
Optimization and variants	Quantitative polymerase chain reaction (qPCR) Reverse transcription polymerase chain reaction (RT-PCR) Inverse polymerase chain reaction (inverse PCR) Nested polymerase chain reaction (nested PCR) Touchdown polymerase chain reaction Hot start PCR Overlap extension polymerase chain reaction (OE-PCR) Multiplex polymerase chain reaction (multiplex PCR) Multiplex ligation-dependent probe amplification (MLPA) Co-amplification at lower denaturation temperature PCR (COLD-PCR) Digital polymerase chain reaction (dePCR) Random amplification of polymorphic DNA (RAPD)
History and people	Kary Mullis Kjell Kleppe Alice Chien